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June 2023
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Flowjo 10 offset plot1/3/2023 ![]() ![]() The system is equipped with a Sutter lambda 10-3 controller, ASI MS2 XY stage with linear encoders, Photometrics CoolSnap HQ CCD camera and Metamorph 7.5 software. If you are using plastic dishes or plates ourĪdvice is to switch off the PFS and use the system in the conventional way. We have used plasticĭishes but have had mixed results. ![]() FlowJo tries to determine which parameter contains the DNA quantitation information if it chooses the wrong one, select the correct one from the X-axis popup menu. You might want to visit the page giving hints for Cell Cycle analysis with FlowJo. The PFS is designed to work with glass bottom dishes. FlowJo brings up a graph window that is specially designed for cell cycle analyses. You should no longer have to adjust the focus knob after adding reagents or after stage movement. ![]() The result is an end to out of focus drift. Is turned on the position of the glass bottom dish/plate is detected using aħ77nm infra red light. The PFS continuously corrects the focus at the plane of interest with its continuous optical offset feature. We have 4 Nikon TE 2000 Timelapse microscope systems with PFS. ![]()
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